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Experiment

Last Updated: March 30, 2007

Protein Crystallization Research Facility (PCRF)

Peltier elements provide temperature profiles suitable for target proteins. Monitoring with CCD camera is possible.

The PCRF has six cell cartridges. Since each cell cartridge has a motor drive and Peltier elements, activation and termination timing as well as temperature profiles can be freely designed. An experimental profile appropriate for each protein can be established. A CCD camera enables real-time monitoring of crystal growth.

crystals grown in space

crystals grown on the ground

monitoring image of CCD camera

Cell Cartridge

  • Six cartridges
  • 10 to 16 wells per cartridge
  • 10 to 500 µl per well

Harvesting Methods

  • Vapor Diffusion
  • Batch
  • Membrane
  • Liquid-liquid Diffusion

Temperature Control

  • 0 to 35 deg .C

Electric Interfaces (per cartridge)

  • Thermistors (2)
  • Thermo module (1)
  • Motor (1)
  • Photo Sensor (1)


Basic Specification

PCRF
Item Specification
Cell Cartridge Number of wells 10 to 16 well / Cell Cartridge
Volume of solution 10 to 500 µl
Temperature control 0 to 35 deg .C (each cartridge is independently controlled)
Harvesting method a) Vapor Diffusion. b) Batch. c) Membrane.
d) Liquid-liquid Diffusion.
Observation system Camera 1/2 CCD camera
Light source LED
Resolution More than 40 μm
 
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