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Subject 2:
Polar transport mechanism of Phytohormones in higher plants under microgravity
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Principal Investigator :
Junichi Ueda, Ph.D.
(Osaka Prefecture University)

Objective : Auxin, one of the plant hormones, is characteristics of its ability to move unidirectionally in stems from the tip to base along the vector of gravity, or basipetally, on Earth (This movement is called "auxin polar transport").
This experiment aims to identify the role of gravity in auxin polar transport.

Experiment outline : Seeds of maize and pea are germinated and allowed to grow in orbit. Stem segments cut from the seedlings are put into small plastic tubes containing 14C-labeled IAA in an agar medium at the bottom of the tubes. After an appropriate incubation, the stem segments are frozen and brought back to Earth, where auxin polar transport is measured. Since auxin is labeled with radioisotope, the transport is readily determined. In addition, auxin polar transport activities in stem segments prepared from the seedlings which have already grown on Earth are also measured after launching in order to clarify the effect of the zero-gravity on auxin polar transport by comparing with those on Earth.

(note) Auxin, one of the plant hormones, is a chemical substance that controls the growth and development of plants.

The cut stems are put into contact with an agar medium containing Auxin.

Expected effects : Auxin, which is considered to regulate the growth and development of plants, moves in stem from the tip to base along the vector of gravity on Earth. In this experiment, the data in relation to the regulating mechanism of auxin polar transport by gravity will be obtained as well as basic information of the plant growth under the zero-gravity.



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