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Subject 5:
Gravimorphogenesis of Cucurbitaceae plants: Development of peg cells and graviperception mechanism in cucumber seedlings
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Principal Investigator :
Hideyuki Takahashi, Ph.D.
(Tohoku University)

Objective : Small protuberance called "peg" appear at the transition zone between stem and root of cucumber seedling immediately after germination. By hooking the peg to the seed coat, the cucumber seedling peels away the seed coat.
Since the peg always develops on the lower side of the horizontally positioned seedling, gravity is likely to play a role in its formation of the "peg."
The experiment aims to observe the formation and positioning of the peg under the zero-gravity condition.

Experiment outline : Cucumber seeds are germinated under the zero-gravity condition to observe the peg formation.
The bottom of a plastic container is covered with a highly water-retentive material, and cucumber seeds are buried and fixed in the material. Once this seed bed is carried to outer space, water is fed to the seeds to induce germination. The state of growth of the cucumber is observed, and the seedlings are chemically fixed for the analysis of various biological parameters.

The cucumber form "peg" during germination. By the peg, the cucumber seedling peels away the seed coat.


Experimental unit : Biological Research in Canisters (BRIC)
In this experiment ,two petri dishes will be stored in a BRIC.

Expected effects : The experiment will clarify the role of gravity in the peg-formation of cucurbitaceous plants such as cucumbers. It will also enable further study on the mechanism of peg formation as well as the formulation of a new experimental model to understand the relation between plants and gravity. These studies will also help to understand why only the cucurbitaceous plants form peg, and how the Earth's gravity affects the evolution of plants. The findings should provide fundamental knowledge for growing plants in outer space in the future.



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